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target regions  (Addgene inc)


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    Addgene inc target regions
    Target Regions, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ar+gfp+plasmid/pmc10208957__41380_2023_1946_MOESM1_ESM-34-21-41?v=Addgene+inc
    Average 93 stars, based on 2 article reviews
    target regions - by Bioz Stars, 2026-07
    93/100 stars

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    Figure 1. ADAR1 depletion is lethal in non-permissive culturing conditions. ( A ) Population doublings (h) of TBP MEFs transduced with non-targeting (NT) or Adar1 sgRNAs clones grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 6 independent measurements. Error bars indicate standard de viation. ( B ) R epresentativ e image of 300.000 serum-starved TBP MEFs from (A) cells cultured for 10 days in 6-well plates. ( C ) Population doublings (h) of TBP ARPE cells transduced with a NT or ADAR1 shRNAs in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 3 independent measurements. Error bars indicate standard deviation. ( D ) Representative image of 25.0 0 0 serum-st arv ed TBP ARPEs from (C) gro wn f or 14 da y s in 6-w ell plates. ( E ) P opulation doublings (h) of NT or AD AR1 knockout TBP MEF reconstituted with linearized pmGFP (vector only), <t>pmGFP-ADAR-p110</t> or pmGFP-ADAR-p150. Cells were grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent six independent measurements. Error bars indicate standard deviation. ( F ) Fraction of Zombie + TBP MEFs from ( E ) cultured in unperturbed (+10% FCS) or serum-starved culturing conditions for 7 days. Dots represent three individual experiments. Error bars indicate standard deviation. Asterisks represent adjusted P -value of two-sided two-way ANO V A (Šidák multiple comparison test) (**** P -value < 0.0 0 01). ns = non-significant. ( G ) Population doublings (h) of NT or ADAR knockdown TBP ARPE cells reconstituted with linearized pmGFP (vector only), pmGFP-ADAR-p110, pmGFP-ADAR-p150, pmGFP-ADAR-p1 50-E91 2A or pmGFP-ADAR-p150-Z αmut grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 4–7 independent measurements. Error bars indicate standard deviation. ( H ) R epresentativ e images of 25.0 0 0 serum st arv ed TBP ARPE cells from (G) cultured f or 14 da y s in 6-w ell plates.
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    Figure 1. ADAR1 depletion is lethal in non-permissive culturing conditions. ( A ) Population doublings (h) of TBP MEFs transduced with non-targeting (NT) or Adar1 sgRNAs clones grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 6 independent measurements. Error bars indicate standard de viation. ( B ) R epresentativ e image of 300.000 serum-starved TBP MEFs from (A) cells cultured for 10 days in 6-well plates. ( C ) Population doublings (h) of TBP ARPE cells transduced with a NT or ADAR1 shRNAs in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 3 independent measurements. Error bars indicate standard deviation. ( D ) Representative image of 25.0 0 0 serum-st arv ed TBP ARPEs from (C) gro wn f or 14 da y s in 6-w ell plates. ( E ) P opulation doublings (h) of NT or AD AR1 knockout TBP MEF reconstituted with linearized pmGFP (vector only), <t>pmGFP-ADAR-p110</t> or pmGFP-ADAR-p150. Cells were grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent six independent measurements. Error bars indicate standard deviation. ( F ) Fraction of Zombie + TBP MEFs from ( E ) cultured in unperturbed (+10% FCS) or serum-starved culturing conditions for 7 days. Dots represent three individual experiments. Error bars indicate standard deviation. Asterisks represent adjusted P -value of two-sided two-way ANO V A (Šidák multiple comparison test) (**** P -value < 0.0 0 01). ns = non-significant. ( G ) Population doublings (h) of NT or ADAR knockdown TBP ARPE cells reconstituted with linearized pmGFP (vector only), pmGFP-ADAR-p110, pmGFP-ADAR-p150, pmGFP-ADAR-p1 50-E91 2A or pmGFP-ADAR-p150-Z αmut grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 4–7 independent measurements. Error bars indicate standard deviation. ( H ) R epresentativ e images of 25.0 0 0 serum st arv ed TBP ARPE cells from (G) cultured f or 14 da y s in 6-w ell plates.
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    Figure 1. ADAR1 depletion is lethal in non-permissive culturing conditions. ( A ) Population doublings (h) of TBP MEFs transduced with non-targeting (NT) or Adar1 sgRNAs clones grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 6 independent measurements. Error bars indicate standard de viation. ( B ) R epresentativ e image of 300.000 serum-starved TBP MEFs from (A) cells cultured for 10 days in 6-well plates. ( C ) Population doublings (h) of TBP ARPE cells transduced with a NT or ADAR1 shRNAs in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 3 independent measurements. Error bars indicate standard deviation. ( D ) Representative image of 25.0 0 0 serum-st arv ed TBP ARPEs from (C) gro wn f or 14 da y s in 6-w ell plates. ( E ) P opulation doublings (h) of NT or AD AR1 knockout TBP MEF reconstituted with linearized pmGFP (vector only), <t>pmGFP-ADAR-p110</t> or pmGFP-ADAR-p150. Cells were grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent six independent measurements. Error bars indicate standard deviation. ( F ) Fraction of Zombie + TBP MEFs from ( E ) cultured in unperturbed (+10% FCS) or serum-starved culturing conditions for 7 days. Dots represent three individual experiments. Error bars indicate standard deviation. Asterisks represent adjusted P -value of two-sided two-way ANO V A (Šidák multiple comparison test) (**** P -value < 0.0 0 01). ns = non-significant. ( G ) Population doublings (h) of NT or ADAR knockdown TBP ARPE cells reconstituted with linearized pmGFP (vector only), pmGFP-ADAR-p110, pmGFP-ADAR-p150, pmGFP-ADAR-p1 50-E91 2A or pmGFP-ADAR-p150-Z αmut grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 4–7 independent measurements. Error bars indicate standard deviation. ( H ) R epresentativ e images of 25.0 0 0 serum st arv ed TBP ARPE cells from (G) cultured f or 14 da y s in 6-w ell plates.
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    Figure 1. ADAR1 depletion is lethal in non-permissive culturing conditions. ( A ) Population doublings (h) of TBP MEFs transduced with non-targeting (NT) or Adar1 sgRNAs clones grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 6 independent measurements. Error bars indicate standard de viation. ( B ) R epresentativ e image of 300.000 serum-starved TBP MEFs from (A) cells cultured for 10 days in 6-well plates. ( C ) Population doublings (h) of TBP ARPE cells transduced with a NT or ADAR1 shRNAs in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 3 independent measurements. Error bars indicate standard deviation. ( D ) Representative image of 25.0 0 0 serum-st arv ed TBP ARPEs from (C) gro wn f or 14 da y s in 6-w ell plates. ( E ) P opulation doublings (h) of NT or AD AR1 knockout TBP MEF reconstituted with linearized pmGFP (vector only), <t>pmGFP-ADAR-p110</t> or pmGFP-ADAR-p150. Cells were grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent six independent measurements. Error bars indicate standard deviation. ( F ) Fraction of Zombie + TBP MEFs from ( E ) cultured in unperturbed (+10% FCS) or serum-starved culturing conditions for 7 days. Dots represent three individual experiments. Error bars indicate standard deviation. Asterisks represent adjusted P -value of two-sided two-way ANO V A (Šidák multiple comparison test) (**** P -value < 0.0 0 01). ns = non-significant. ( G ) Population doublings (h) of NT or ADAR knockdown TBP ARPE cells reconstituted with linearized pmGFP (vector only), pmGFP-ADAR-p110, pmGFP-ADAR-p150, pmGFP-ADAR-p1 50-E91 2A or pmGFP-ADAR-p150-Z αmut grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 4–7 independent measurements. Error bars indicate standard deviation. ( H ) R epresentativ e images of 25.0 0 0 serum st arv ed TBP ARPE cells from (G) cultured f or 14 da y s in 6-w ell plates.
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    Image Search Results


    Figure 1. ADAR1 depletion is lethal in non-permissive culturing conditions. ( A ) Population doublings (h) of TBP MEFs transduced with non-targeting (NT) or Adar1 sgRNAs clones grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 6 independent measurements. Error bars indicate standard de viation. ( B ) R epresentativ e image of 300.000 serum-starved TBP MEFs from (A) cells cultured for 10 days in 6-well plates. ( C ) Population doublings (h) of TBP ARPE cells transduced with a NT or ADAR1 shRNAs in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 3 independent measurements. Error bars indicate standard deviation. ( D ) Representative image of 25.0 0 0 serum-st arv ed TBP ARPEs from (C) gro wn f or 14 da y s in 6-w ell plates. ( E ) P opulation doublings (h) of NT or AD AR1 knockout TBP MEF reconstituted with linearized pmGFP (vector only), pmGFP-ADAR-p110 or pmGFP-ADAR-p150. Cells were grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent six independent measurements. Error bars indicate standard deviation. ( F ) Fraction of Zombie + TBP MEFs from ( E ) cultured in unperturbed (+10% FCS) or serum-starved culturing conditions for 7 days. Dots represent three individual experiments. Error bars indicate standard deviation. Asterisks represent adjusted P -value of two-sided two-way ANO V A (Šidák multiple comparison test) (**** P -value < 0.0 0 01). ns = non-significant. ( G ) Population doublings (h) of NT or ADAR knockdown TBP ARPE cells reconstituted with linearized pmGFP (vector only), pmGFP-ADAR-p110, pmGFP-ADAR-p150, pmGFP-ADAR-p1 50-E91 2A or pmGFP-ADAR-p150-Z αmut grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 4–7 independent measurements. Error bars indicate standard deviation. ( H ) R epresentativ e images of 25.0 0 0 serum st arv ed TBP ARPE cells from (G) cultured f or 14 da y s in 6-w ell plates.

    Journal: Nucleic acids research

    Article Title: ADARp150 counteracts whole genome duplication.

    doi: 10.1093/nar/gkae700

    Figure Lengend Snippet: Figure 1. ADAR1 depletion is lethal in non-permissive culturing conditions. ( A ) Population doublings (h) of TBP MEFs transduced with non-targeting (NT) or Adar1 sgRNAs clones grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 6 independent measurements. Error bars indicate standard de viation. ( B ) R epresentativ e image of 300.000 serum-starved TBP MEFs from (A) cells cultured for 10 days in 6-well plates. ( C ) Population doublings (h) of TBP ARPE cells transduced with a NT or ADAR1 shRNAs in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 3 independent measurements. Error bars indicate standard deviation. ( D ) Representative image of 25.0 0 0 serum-st arv ed TBP ARPEs from (C) gro wn f or 14 da y s in 6-w ell plates. ( E ) P opulation doublings (h) of NT or AD AR1 knockout TBP MEF reconstituted with linearized pmGFP (vector only), pmGFP-ADAR-p110 or pmGFP-ADAR-p150. Cells were grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent six independent measurements. Error bars indicate standard deviation. ( F ) Fraction of Zombie + TBP MEFs from ( E ) cultured in unperturbed (+10% FCS) or serum-starved culturing conditions for 7 days. Dots represent three individual experiments. Error bars indicate standard deviation. Asterisks represent adjusted P -value of two-sided two-way ANO V A (Šidák multiple comparison test) (**** P -value < 0.0 0 01). ns = non-significant. ( G ) Population doublings (h) of NT or ADAR knockdown TBP ARPE cells reconstituted with linearized pmGFP (vector only), pmGFP-ADAR-p110, pmGFP-ADAR-p150, pmGFP-ADAR-p1 50-E91 2A or pmGFP-ADAR-p150-Z αmut grown in unperturbed culturing conditions (+10% FCS) and passed before reaching confluency. Dots represent 4–7 independent measurements. Error bars indicate standard deviation. ( H ) R epresentativ e images of 25.0 0 0 serum st arv ed TBP ARPE cells from (G) cultured f or 14 da y s in 6-w ell plates.

    Article Snippet: ADARp110 and ADARp150 cDNA was reconstituted into TBP cells by transfecting Pvu1-linearized pm-GFP-AD AR-p110 (Addgene #117928), pmGFP-AD ARp150 (Addgene #117927) or control pmGFP (Addgene #117926) (vector only).

    Techniques: Transduction, Clone Assay, Cell Culture, Standard Deviation, Knock-Out, Plasmid Preparation, Comparison, Knockdown